PDK4 polyclonal antibody
产品名称： PDK4 polyclonal antibody
英文名称： PDK4 polyclonal antibody
产品编号： PAB4564
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PDK4.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulation of the PDK4 isozyme by the Rb-E2F1 complex.
Hsieh MC, Das D, Sambandam N, Zhang MQ, Nahle Z.J Biol Chem. 2008 Oct 10;283(41):27410-7. Epub 2008 Jul 30.
- 2.
- CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress.
Nahle Z, Hsieh M, Pietka T, Coburn CT, Grimaldi PA, Zhang MQ, Das D, Abumrad NA.J Biol Chem. 2008 May 23;283(21):14317-26. Epub 2008 Feb 28.
- 3.
- The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes.
White UA, Coulter AA, Miles TK, Stephens JM.Diabetes. 2007 Jun;56(6):1623-9. Epub 2007 Mar 14.
- Applications
- Western Blot (Tissue lysate)
- The PDK4 polyclonal antibody (Cat # PAB4564) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate .
- Western Blot (Transfected lysate)
- Western blot analysis of PDK4 (arrow) using PDK4 polyclonal antibody (Cat # PAB4564). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK4 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human skeletal musclereacted with PDK4 polyclonal antibody (Cat # PAB4564), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human adrenalreacted with PDK4 polyclonal antibody (Cat # PAB4564), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5166
- Protein Accession#:
- NP_002603;Q16654
- Gene Name:
- PDK4
- Gene Alias:
- FLJ40832
- Gene Description:
- pyruvate dehydrogenase kinase, isozyme 4
- Omim ID:
- 602527
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
- Other Designations:
- pyruvate dehydrogenase kinase 4,pyruvate dehydrogenase kinase, isoenzyme 4
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